Pertussis (pertussis) is a highly contagious acute respiratory disease mainly caused by B. pertussis infection. The cough gradually worsened, showing a typical paroxysmal and spastic cough. At the end of the cough, a deep and long chicken-like inspiratory roar appeared, and the total number of white blood cells and lymphocytes increased. Pertussis can cause a series of serious complications such as pneumonia and pertussis encephalopathy, and endanger the health of newborns.

  Clinically, bacterial culture and serological screening are commonly used to detect B. pertussis, but the isolation and cultivation of B. pertussis is difficult and the culture cycle is long. Serological screening has the defects of low sensitivity and low specificity, which limits the above Clinical application of two detection methods for pertussis bacteria. "Expert Consensus on the Diagnosis and Treatment of Chronic Wet Cough in Chinese Children (2019 Edition)" proposed "Pertussis PCR detection has high sensitivity and can be diagnosed early". Therefore, the detection of pertussis nucleic acid can detect the cause of cough as early as possible; accurately guide the use of antibiotics, reduce drug abuse, and reduce complications; prevent and control infection early.