Fluorescent microsphere labeling technology uses the principle of membrane chromatography double antibody sandwich method to detect influenza A and / or influenza B virus antigens in samples. During the test, the treated samples are added dropwise to the sample wells corresponding to the two detection areas of the test cards A and B. When the influenza A and (or) B viruses are present in the sample, the virus antigen and quantum dot nanospheres After the labeled influenza A and (or) influenza B virus nucleoprotein monoclonal antibody I is combined, when the chromatography line is detected, it will be captured by the coated influenza A and (or) influenza B virus nucleoprotein monoclonal antibody II Gather at the position of the detection line to form a fluorescent band A line and (or) B line. Under ultraviolet irradiation with a wavelength of 365nm, it can be determined by the naked eye that the influenza A and (or) B virus is positive, or by fluorescence The result of the chromatography test strip reader is positive for influenza A and / or B viruses.