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L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)
L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)

L. pneumophila nucleic acid detection kit (PCR-fluorescent probe method)

【Clinical manifestations】
  Approximately 90% of Legionellosis is caused by Legionella pneumophila. The causative agent of Legionella pneumonia is Legionella pneumophila, which accounts for 2 to 5% of community-acquired pneumonia . The symptoms of Legionella disease vary widely, from common cold and low fever to high fever, with changes in mental status and respiratory failure; atypical symptoms usually occur early in the disease, including headache, muscle aches, anorexia, fatigue, etc. . Diarrhea and other gastrointestinal symptoms are approximately 20-40% . Among the atypical cases of community-acquired pneumonia, Legionella disease is the most serious clinically, and if not treated properly and in time, it will progressively worsen. Although there are few extrapulmonary symptoms, it can also cause myocarditis, pericarditis and artificial valve endocarditis, as well as glomerulonephritis, pancreatitis and peritonitis.
【Features】
  This kit uses real-time fluorescent PCR technology, takes the highly conserved region of the Legionella pneumophila genome as the target region, designs specific primers and fluorescent probes, and then performs PCR amplification on the target sequence , while monitoring real-time expansion through Taqman fluorescent probe technology. Accumulation of augmentation products enables qualitative detection of Norovirus nucleic acids in samples . The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincidence rate is 100%

Precision

Coefficient of variation within and between batches CV≤5%

Specificity

100% specificity , no cross reaction with Staphylococcus aureus, 

respiratory syncytial virus, Epstein-Barr virus, Haemophilus influenzae, 

Mycoplasma pneumoniae, Streptococcus pneumoniae, etc.

Anti-interference

Oxymetazoline hydrochloride spray, budesonide nasal spray, erythromycin, 

roxithromycin, azithromycin, etc. did not significantly interfere with the test results

【Product information】
Test specimen: throat swab
Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche LightCycler 480 ; ABI 7500 ; Shanghai Hongshi SLAN-96S ; Bioer LineGene 9600, etc.