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Norovirus nucleic acid detection kit (PCR-fluorescent probe method)
Norovirus nucleic acid detection kit (PCR-fluorescent probe method)

Norovirus nucleic acid detection kit (PCR-fluorescent probe method)

【Clinical manifestations】

  Norovirus  is considered to be the major cause of non-bacterial pathogen outbreaks of acute gastroenteritis, often causing vomiting, diarrhea and other symptoms, can be transmitted through a variety of ways food, water and so on. Norovirus virus divided . 5 genome, wherein G Ⅰ, G Ⅱ Norovirus is the most common worldwide genome, reported to have a global epidemic diffusion. Norovirus is mainly infected through the fecal-oral route. After infection, humans and animals can secrete a large number of norovirus particles. Innovirus has strong viability and infectivity in vitro. Norovirus can be infected in small doses (<100 virus particles), and the population is generally susceptible to it. The clinical symptoms of the human body after being infected with Norovirus are mainly vomiting and diarrhea, and it is difficult to distinguish the symptoms of gastroenteritis caused by other pathogens.

【Features】
  This kit utilizes real-time fluorescence PCR technology to norovirus G Ⅰ, G Ⅱ type genomic region is highly conserved target region, the design of specific primers and fluorescent probes, and target sequences for PCR amplification, through Taqman fluorescent probes Needle technology monitors the accumulation of amplification products in real time, enabling qualitative detection of Norovirus nucleic acids in samples . The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results .
【Product Performance】

Sensitivity

1.0x103copies/mL

Linear range

1.0x103copies/mL ~ 1.0x 109copies/mL

Accuracy

The detection result coincidence rate is 100%

Precision

Coefficient of variation within and between batches CV≤5%

Specificity

A specificity of 100% , with the wheel-like virus, Sapporo virus, 

enteric adenovirus virus, stellate virus, Salmonella, 

Shigella and other non-cross-reactive

Anti-interference

10% pus blood, 0.5% erythromycin suppository, 

0.5% anal Thai suppository, etc. did not significantly interfere with the test results

【product information】
Test specimens: feces, anal swabs
Technical principle: PCR- fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Applicable instruments: Roche Cobas z 480 ; Roche LightCycler 480 ; ABI 7500 ; Shanghai Hongshi SLAN-96S ; Bioer LineGene 9600, etc.