Human metapneumovirus nucleic acid detection kit (PCR-fluorescent probe method)

【Clinical manifestations】

Human metapneumovirus is a new human respiratory virus discovered by Dutch scholars in 2001 in children with unexplained respiratory infections. Infants, the elderly, and immunodeficiency patients have a higher incidence, causing clinical symptoms similar to respiratory syncytial virus. Common symptoms after human metapneumovirus infection include runny nose, cough, fever, and difficulty breathing. Laryngitis and other upper respiratory tract symptoms, bronchiolitis, pneumonia and other lower respiratory tract symptoms, combined with membranitis, otitis media, etc.

【Features】

This kit uses real-time fluorescent PCR technology, takes the highly conserved region of the human metapneumovirus genome as the target region, designs specific primers and fluorescent probes, and then performs PCR amplification on the target sequence , while real-time monitoring and expansion through Taqman fluorescent probe technology. Accumulation of augmentation products enables qualitative detection of respiratory syncytial virus nucleic acid in the sample. The reaction system of this kit contains dU-UNG enzyme anti-pollution system to avoid false positive results.

【Product Performance】

Sensitivity	1.0x10³copies/mL
Linear range	1.0x10³copies/mL ~ 1.0x 10⁹copies/mL
Accuracy	The detection result coincidence rate is 100%
Precision	Coefficient of variation within and between batches CV≤5%
Specificity	100% specificity , no cross reaction with influenza A virus, influenza B virus, adenovirus , human Boca virus, Epstein-Barr virus, Mycoplasma pneumoniae, Chlamydia pneumoniae, etc.
Anti-interference	Oxymetazoline hydrochloride spray, budesonide nasal spray , zanamivir , ribavirin did not interfere with the test results