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Taqman one-step real-time fluorescence RT-PCR detection kit
Taqman one-step real-time fluorescence RT-PCR detection kit

Taqman one-step real-time fluorescence RT-PCR detection kit

Product Brief

   Taqman one-step real-time fluorescence RT-PCR reagents include 25 × RT-PCR mixed enzymes, 2 × OneStep RT-PCR Buffer and RNA / DNase-free water. It is a special reagent for Real Time One Step RT-PCR by hydrolysis probe method After adding the template and primer probes, one-step method can achieve high efficiency of reverse transcription and PCR amplification in one tube. It is also suitable for Roche PCR amplifiers that use glass capillaries and does not require the addition of BSA. This product can also be used for rapid Real Time PCR amplification reactions. This product can get a good standard curve in a wide quantitative area, accurate quantitative detection of target genes, good repeatability and high reliability.

Product advantages
1. There is no need to open the lid during the RNA amplification process, so there is less pollution.
2. Reagents are spare type, just add template and primer probe, easy to operate.
3. There is no preference in the reverse transcription process. Simultaneous reverse transcription of high-abundance and low-abundance RNA in the same tube can improve the sensitivity and accuracy of the detection and the repeatability is good.
4. Strong applicability, whether it is a high GC ratio template or RNA with a complicated secondary structure, can easily perform reverse transcription and PCR.
5. A good standard curve can be obtained in a wide quantitative area, and experimental verification can obtain a standard curve of at least 9 orders of magnitude, and even a small amount of template can be accurately quantitatively detected.
6. The reagent contains Rox fluorescence correction, eliminates the difference between the detected wells, and can be used to standardize the quantitative fluorescence signal




The Taqman one-step real-time fluorescent RT-PCR detection kit is used for quantitative quantitative analysis of the target gene for accurate quantitative detection, with good repeatability and high reliability.



Applicable instruments

Fluorescence quantitative PCR instrument