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Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)
Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)

Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)

Clinical background

  Adenovirus (ADV) is a group of very widely distributed viruses with double helix DNA genomes. Acute infectious diseases caused by ADV can easily invade the mucous membranes of the respiratory tract and digestive tract, ocular conjunctiva, urinary tract and lymph nodes, mainly manifested as acute upper respiratory tract. Infection (2% to 4% of acute respiratory infections caused by adenovirus), followed by eye and gastrointestinal infections. The population is generally susceptible, more common in children, about half of the patients are hidden infections. Infants and young children are susceptible to adenovirus pneumonia, which accounts for about 10% of childhood pneumonia. The condition is serious and the mortality is high. The mortality of adenovirus pneumonia in young people is 8% to 10%, and there is no specific treatment. According to the investigation of serum specific antibody and virus isolation, it can be seen that adenovirus infection is very extensive.

Precision and linear range


Figure 1: Precision (using 1.0x104PFU / mL reference material as a template, repeated 20 times in the same batch to detect CV value ≤5%)
Figure 2: Linear range (for 103 ~ 108PFU / mL concentration range reference products, the product amplification curve is smooth and complete, the correlation coefficient between different gradients is R> 99%, and the correlation is good)



Product performance

Adenovirus nucleic acid detection kit (PCR-fluorescent probe method)

Sensitivity

1.0x103PFU/mL

Linear range

1.0x103PFU/mL ~ 1.0x 108PFU/mL

Accuracy

The positive coincidence rate was 100%, the negative coincidence rate was 98.83%, and the overall coincidence rate was 99.41%

Precision

Coefficient of variation within and between batches CV≤5%

Specificity

100% specificity, no cross reaction with other respiratory pathogens

Anti-interference

Mucin, blood, pus, erythromycin, chloramphenicol did not interfere with the test results

product information
Test specimen: throat swab
Technical principle: PCR-fluorescent probe method
Packaging specifications: 24 tests/ kit, 48 tests/ kit, 96 tests/ kit
Category: In vitro diagnostic reagents
Applicable instruments: fluorescence quantitative PCR instrument