Adenovirus (ADV) is a group of very widely distributed viruses with double helix DNA genomes. Acute infectious diseases caused by ADV can easily invade the mucous membranes of the respiratory tract and digestive tract, ocular conjunctiva, urinary tract and lymph nodes, mainly manifested as acute upper respiratory tract. Infection (2% to 4% of acute respiratory infections caused by adenovirus), followed by eye and gastrointestinal infections. The population is generally susceptible, more common in children, about half of the patients are hidden infections. Infants and young children are susceptible to adenovirus pneumonia, which accounts for about 10% of childhood pneumonia. The condition is serious and the mortality is high. The mortality of adenovirus pneumonia in young people is 8% to 10%, and there is no specific treatment. According to the investigation of serum specific antibody and virus isolation, it can be seen that adenovirus infection is very extensive.
Figure 1: Precision (using 1.0x104PFU / mL reference material as a template, repeated 20 times in the same batch to detect CV value ≤5%)
Figure 2: Linear range (for 103 ~ 108PFU / mL concentration range reference products, the product amplification curve is smooth and complete, the correlation coefficient between different gradients is R> 99%, and the correlation is good)
Adenovirus nucleic acid detection kit (PCR-fluorescent probe method) |
|
Sensitivity |
1.0x103PFU/mL |
Linear range |
1.0x103PFU/mL ~ 1.0x 108PFU/mL |
Accuracy |
The positive coincidence rate was 100%, the negative coincidence rate was 98.83%, and the overall coincidence rate was 99.41% |
Precision |
Coefficient of variation within and between batches CV≤5% |
Specificity |
100% specificity, no cross reaction with other respiratory pathogens |
Anti-interference |
Mucin, blood, pus, erythromycin, chloramphenicol did not interfere with the test results |